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FUJIFILM eagle’s minimum essential medium (e-mem)
Eagle’s Minimum Essential Medium (E Mem), supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eagle%E2%80%99s+minimum+essential+medium+(e-mem)/eagle%E2%80%99s+minimum+essential+medium/pm40286947-59-28-33
Average 90 stars, based on 1 article reviews
eagle’s minimum essential medium (e-mem) - by Bioz Stars, 2026-10
90/100 stars

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Cell Culture:

Article Title: Analyses of cell death mechanisms related to amino acid substitution at position 95 in the rabies virus matrix protein.
Article Snippet: Received 04 December 2020; Accepted 09 April 2021; Published 23 April 2021 Author affiliations: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Joint Graduate School of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Joint Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.. *Correspondence: Tatsunori Masatani, mstn@ gifuu. ac. jp

Article Title: Assessment of Radiolabelled Derivatives of R954 for Detection of Bradykinin B1 Receptor in Cancer Cells: Studies on Glioblastoma Xenografts in Mice
Article Snippet: Human glioblastoma cell lines U87MG and U251MG were obtained from the American Type Culture Collection (Manassas, VA, USA) and kept under standard cell culture conditions (5% CO 2 , 37 °C). .. Cells were cultured in Eagle’s minimum essential medium (E-MEM; FUJIFILM Wako Pure Chemical Co.) supplemented with 10% v / v foetal bovine serum (BioWest S.A.S, Nuaillé, France), 1% v / v nonessential amino acids (FUJIFILM Wako Pure Chemical Co.), 1 mM sodium pyruvate (FUJIFILM Wako Pure Chemical Co.), and 1% v / v penicillin–streptomycin (10,000 unit-10 mg/mL; FUJIFILM Wako Pure Chemical Co.). .. Cells were seeded at a density of 1 × 10 5 cells/well in Poly- d -Lysine 24-well plates (Corning Inc., Corning, NY, USA).

Article Title: HMGA2 directly mediates chromatin condensation in association with neuronal fate regulation
Article Snippet: .. IMR90 cells and Neuro2A cells were cultured under 5% CO 2 at 37 °C in Eagle’s minimum essential medium (E-MEM, Fujifilm) or DMEM (Fujifilm), respectively, each supplemented with 10% fetal bovine serum (JRH Bioscience or Gibco) and 1% penicillin-streptomycin (Invitrogen). .. Cells were seeded in 24-well plates (5 × 10 3 cells per well) and cultured for 1 day before transfection with the use of polyethyleneimine (Polysciences), GeneJuice (Millipore) or Lipofectamine LTX (Thermo Fisher) reagents.

Article Title: Isolation and characterization of Iwanai Valley virus, a new tick-borne nairovirus from Ixodes ovatus ticks in Hokkaido, Japan.
Article Snippet: Most members of the genus Orthonairovirus, represented by Crimean-Congo hemorrhagic fever virus and Nairobi sheep disease virus, are tick-borne, and some have become a public health concern in recent years.. Here, we report the isolation and genetic and biological characterization of a new orthonairovirus, designated as "Iwanai Valley virus" (IWVV), from Ixodes ovatus ticks in Hokkaido, Japan.. The amino acid sequence of the viral nucleoprotein (NP) was found to be 34–45% identical to those of known orthonairoviruses.

Article Title: Assessment of Radiolabelled Derivatives of R954 for Detection of Bradykinin B1 Receptor in Cancer Cells: Studies on Glioblastoma Xenografts in Mice.
Article Snippet: Human glioblastoma cell lines U87MG and U251MG were obtained from the American Type Culture Collection (Manassas, VA, USA) and kept under standard cell culture conditions (5% CO2, 37 ◦C). .. Cells were cultured in Eagle’s minimum essential medium (E-MEM; FUJIFILM Wako Pure Chemical Co.) supplemented with 10% v/v foetal bovine serum (BioWest S.A.S, Nuaillé, France), 1% v/v nonessential amino acids (FUJIFILM Wako Pure Chemical Co.), 1 mM sodium pyruvate (FUJIFILM Wako Pure Chemical Co.), and 1% v/v penicillin–streptomycin (10,000 unit-10 mg/mL; FUJIFILM Wako Pure Chemical Co.). .. Cells were seeded at a density of 1 × 105 cells/well in Poly-D-Lysine 24-well plates (Corning Inc., Corning, NY, USA).

Article Title: Effect of differently coated titanium dioxide nanoparticles on the lung in wild-type and Nrf2 null mice
Article Snippet: Human lung carcinoma A549 cells were obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). .. The A549 cells were cultured in Eagle’s Minimum Essential Medium (E-MEM, FUJIFILM Wako Pure Chemical) supplemented with 10% fetal bovine serum (FBS, Biowest, Riverside, MO) and MEM non-essential amino acids (MEM NEAA, Thermo Fisher Scientific) at 37°C in an atmosphere of 5% CO2. .. The A549 cells were seeded in T25 flasks (Sumitomo Bakelite Co, Tokyo, Japan) and passaged every 2–3 days.

Article Title: HMGA2 directly mediates chromatin condensation in association with neuronal fate regulation.
Article Snippet: .. IMR90 cells and Neuro2A cells were cultured under 5% CO2 at 37 °C in Eagle’s minimum essential medium (E-MEM, Fujifilm) or DMEM (Fujifilm), respectively, each supplemented with 10% fetal bovine serum (JRH Bioscience or Gibco) and 1% penicillin-streptomycin (Invitrogen). .. Cells were seeded in 24-well plates (5 × 103 cells per well) and cultured for 1 day before transfection with the use of polyethyleneimine (Polysciences), GeneJuice (Millipore) or Lipofectamine LTX (Thermo Fisher) reagents.

Derivative Assay:

Article Title: Isolation and characterization of Iwanai Valley virus, a new tick-borne nairovirus from Ixodes ovatus ticks in Hokkaido, Japan.
Article Snippet: Most members of the genus Orthonairovirus, represented by Crimean-Congo hemorrhagic fever virus and Nairobi sheep disease virus, are tick-borne, and some have become a public health concern in recent years.. Here, we report the isolation and genetic and biological characterization of a new orthonairovirus, designated as "Iwanai Valley virus" (IWVV), from Ixodes ovatus ticks in Hokkaido, Japan.. The amino acid sequence of the viral nucleoprotein (NP) was found to be 34–45% identical to those of known orthonairoviruses.

other:

Article Title: Development of antitumor biguanides targeting energy metabolism and stress responses in the tumor microenvironment
Article Snippet: Eagle’s minimum essential medium (E-MEM, 051-07615), 1 mmol/L sodium pyruvate, RPMI-1640 (with 2000 mg/L of glucose, 189-02025), Sodium Dodecyl Sulfate (SDS, 194-13985), Tween 20, bovine serum albumin (5217/100G) and 45w/v% D(+)-Glucose Solution (079-05511) were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).



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